Unite Union members will walk out from Sunday to Wednesday.
The service carries 22 million passengers a year.
But around 60 members of staff say they are angry their colleagues on the London Underground are paid up to £10,000 more a year.
They say that’s despite requiring the same qualifications and performing the same roles.
Navid Golshan, General Manager for London Trams, said: “We urge Unite the Union to continue to work with us to find a resolution and call this strike off. Customers planning to use London Trams services are urged to check before they travel, allow extra time for their journeys, and check the TfL website or the TfL Go app for the latest travel information.”
TfL staff and Travel Ambassadors will be deployed on the busiest parts of the London Trams network to assist customers with their onward journeys.
These locations include Addington Village, Beckenham Junction, East Croydon, Elmers End, West Croydon and Wimbledon.
